Expression of LIMS-1 Protein in Selected Cancers of the Genital Organ in Women (Pages 65-70)
Paweł Madej1, Janusz Antoni Madej2, Grzegorz Franik1, Marcin Nowak2, Rafał Ciaputa2, Krzysztof Pawlicki4, Dariusz Piecha3 and Dagmara Pluta1
1Department of Gynaecological Endocrinology, Medical University of Silesia, 40-752 Katowice, Medyków St. 14, Poland; 2Department of Pathology, Department of Pathomorphology and Forensic Science, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Norwida St. 31, Poland; 3Department of Gynaecology and Obstetrics, St. Luke Hospital in Piekary Śląskie, 41-940 Piekary Śląskie, Szpitalna St. 11, Poland; 4Department of Biophysic, Medical University of Silesia, 40-752 Katowice, Medyków St 16, Poland
Aim: The study aimed at immunohistochemical evaluation of LIMS-1 (PINCH-1-cysteine-histidine-rich) protein expression and localization in squamocellular cancer of uterine cervix, grades IB1 (T1b) and IIIB, in endometrial cancer, grades IB (T2b, NO, MO) and IIIB (T1, T2, T3, N1, MO) and in serous ovarian cancer, grades IB (pT1b) and IIIB (pT3b).
Methods: The immunohistochemical studies were conducted in paraffin sections, using rabbit polyclonal antibodies specific for LIMS-1 (SigmaR), diluted 1/200. The reaction was developed using EnVisionIM FLEX DAB+ Chromogen DAB solution. The expression was evaluated using modified IRS scale according to Remmele. Statistical analysis involved application of Kolmogonov–Smirnov test of normal distribution, Kruskall–Wallis test, analysis of K–means cluster and R-Spearman test.
Results: there was no relationship between the expression of LIMS-1 and the advancement of neoplastic disease according to FIGO classification in squamocellular cancer of uterine cervix: the significance coefficient in the chi-square test amounted to p = 0.086. In cases of endometrial cancer and ovarian cancer, the relationship between these variables showed the level of significance to be p = 0.045 and p = 0.041, respectively. In the cases where the chi-square test demonstrated a relationship between the stage of FIGO classification and the LIMS-1 expression, the R correlation coefficients of Spearman presented values of 0.66 (p=0.003) for endometrial cancer and 0.88 (p=0.005).
Conclusion: Increase in expression level of LIMS-1 protein in studied neoplasms was demonstrated to manifest positive correlation with degree of histological malignancy according to FIGO, or with neoplastic progression.
Keywords: Endometrial cancer, FIGO classification, LIMS-1 (PINCH-1), serous ovarian cancer, squamocellular cancer of uterine cervix.